PTEN or Cilostazol on artificial-induced ovarian development in the Japanese eel

PTEN or Cilostazol on artificial-induced ovarian development

in the Japanese eel,  Anguilla Japonica

 

Yung-Sen Huang¹*, Jin-Chywan Gwo², and Ching-Fong Chang²

¹ Depart. Life. Sci., National University of Kaohsiung;

² Depart. Aquaculture, National Taiwan Ocean University;

yshuang@nuk.edu.tw

 

The stimulatory effects of gonaotropins upon ovarian development are mainly provoked by the intracellular cAMP production through GPCR (G-protein-couple receptors); furthermore, PI3K (phosphoinositide-3-kinases) pathway is now recognized as one critical pathways in early follicular development, roles of the PI3K were revealed by deletion of PTEN (Phosphatase and Tensin homolog on chromosome 10). Two forms of PTEN have been cloned from the Japanese eel. Cilostazol (Pletal®) is a selective inhibitor of phosphodiesterase type III (PDE3, mainly located in mammalian ovarian follicle cells) and Cilostazol-induced vasculo-angiogenesis effects have also been shown, but there is no data on its effects in fish. The natural blockage and inducible of ovarian development in the eel was a benefit to study roles of PTEN and Cilostazol on the induced ovarian growth. Our data suggested that two forms of PTEN are existing in the eel, and progress of ovarian development corresponded to the decrease of ovarian PTEN expression, vice versa. A continuing administration of PDE3 inhibitor had inhibitory effects on the induced ovarian development in the Japanese eel, and intracellular cAMP levels seem to be temporal and spatial regulated in the eel ovarian follicles.  The applications of PTEN-inhibitors and/or PDE3-inhibitors might be useful to get a good quality egg from artificial maturation.  

 

Key words: Anguilla japonica, cAMP, Cilostazol, ovary, PDE3, PI3K, PTEN, T