Proteomics. 2018 Apr;18(8):e1700445. doi: 10.1002/pmic.201700445. Epub 2018 Mar 30.
Proteomic Studies on the Swim Bladder of the European Eel (Anguilla anguilla)
Abstract
The swim bladder of a fish is a vital organ that with gas gland cells in the swim bladder wall enables key physiological functions including buoyancy regulation in the face of different hydrostatic pressures. Specific gas gland cells produce and secrete acidic metabolites into the blood in order to reduce the physical solubility of gases and blood gas transport capacity for regulating the volume of the swim bladder. Transcriptomic analyses have provided evidence at the RNA level but no specific studies at the protein level have been carried out so far. Herein, it was the aim of the study to show swim bladder proteins of the yellow stage European eel by label-free LCMS (Q-Exactive Plus) that resulted in the identification of 6223 protein groups. Neurotransmitter receptors and transporters were enriched in the membrane fraction and enzymes for acid production were observed. The list of identified proteins may represent a useful tool for further proteomics experiments on this organ. All MS proteomics data are available at the PRIDE repository with the dataset identifier PXD007850.
KEYWORDS:
Neurotransmitter; Receptors; Swim bladder; transporters
https://www.ebi.ac.uk/pride/archive/projects/PXD007850/files
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