cilostazol 對鰻魚人工催熟卵巢初期發育的影響

發表於 2014 年 The Asia and Oceania Society for the Comparative Endocrinology (AOSCE) 會議

 

 

Effects of the Cilostazol (a specific phosphodiesterase type Ⅲ inhibitor) on primary-secondary oocyte development in the induced Japanese eel

 

Zhu C.-W. ¹, Lee Y.-H. ², Chang C.-F. ³ and Huang Y.-S.¹*

1Depart. Life Sci., Natl. Univ. of Kaoshiung, Taiwan. 2 Tungkang Biotech. Res. Center, TFRI., Taiwan. 3 Depart, Aquaculture, Natl. Taiwan Ocean Univ., Keelung, Taiwan.

e-mail: yshuang@nuk.edu.tw

 

The stimulatory effects of gonadotropins upon ovarian development are provoked by the intracellular cAMP production in mammals. Cilostazol (Pletal®) is a selective inhibitor of phosphodiesterase type III (PDE3, mainly located in mammalian ovarian follicle cells) and mediates its effects by increasing cellular cAMP levels. Furthermore, cilostazol-induced vasculo-angiogenesis effects have also been shown, but there is no data on its effects in fish. We address this question in the Japanese eel, since its ovarian development could be manipulated to study if cilostazol can ameliorate induced ovarian development or reinforce ovarian angiogenesis. Eels were treated with salmon pituitary extracts (SPE), cilostazol (cilo), or the combination (SPE+cilo) twice a week for 6 weeks. In the end, the ovarian tissues, gas glands, and blood of treated eels were sampled. The data showed that the GSI (gonadal index) and RMSI (gas glands index) were both stimulated by SPE while the levels of blood glucose (BG) significantly (x2.5) stimulated in the SPE+cilo group uniquely as compared with the controls; the positive effect of SPE or cilo alone on RMSI was showed, an added positive effect on RMSI was seen in the SPE+cilo group; the positive effect of SPE on GSI seemed inhibited by cilo, more interesting, cilo alone inhibited GSI significantly (x0.75) as compared with the controls. The distributions of oocyte diameters in each group were tested, cilo alone inhibited maximal oocyte diameters as compared with the controls, and the positive effect of SPE on maximal oocyte diameters was also inhibited by cilo. Our results suggested that a continuing administration of PDE3 inhibitor had inhibitory effects on the induced ovarian development in the Japanese eel, and intracellular cAMP levels seem to be temporal and spatial regulated in the eel ovarian follicles.                         

 

Keyword: Anguilla japonica, development, oocyte, phosphodiesterase inhibitor,