PTEN or Cilostazol on
artificial-induced ovarian development
in the Japanese eel, Anguilla Japonica
Yung-Sen Huang1*,
Jin-Chywan Gwo2, and Ching-Fong Chang2
1 Depart. Life. Sci., National
University of Kaohsiung;
2 Depart. Aquaculture, National
Taiwan Ocean
University;
yshuang@nuk.edu.tw
The
stimulatory effects of gonaotropins upon ovarian development are mainly provoked
by the intracellular cAMP production through GPCR (G-protein-couple
receptors); furthermore, PI3K
(phosphoinositide-3-kinases) pathway is now recognized as one critical pathways
in early follicular development, roles of the PI3K were revealed by deletion of
PTEN (Phosphatase and Tensin homolog
on chromosome 10). Two forms of PTEN have been cloned from the Japanese eel. Cilostazol (Pletal®)
is a selective inhibitor of phosphodiesterase type III (PDE3, mainly located in
mammalian ovarian follicle cells) and Cilostazol-induced vasculo-angiogenesis
effects have also been shown, but there is no data on its effects in fish. The
natural blockage and inducible of ovarian development in the eel was a benefit
to study roles of PTEN and Cilostazol on the induced ovarian growth. Our data
suggested that two forms of PTEN are existing in the eel, and progress of ovarian
development corresponded to the decrease of ovarian PTEN expression, vice versa. A
continuing administration of PDE3
inhibitor had inhibitory effects on the induced ovarian development in the
Japanese eel, and intracellular cAMP levels seem to be temporal and spatial
regulated in the eel ovarian follicles. The
applications of PTEN-inhibitors and/or PDE3-inhibitors might be useful to get a
good quality egg from artificial maturation.
Key words: Anguilla japonica,
cAMP, Cilostazol, ovary, PDE3, PI3K, PTEN, T
No comments:
Post a Comment