Abstract
Androgens stimulate ovarian development in
eels. Our previous report indicated a correlation between the initial (debut)
ovarian status (determined by kernel density estimation (KDE), presented as a
probability density of oocyte size) and the consequence of 17MT treatment
(change in ovary). The initial ovarian status appeared to be an important
factor influencing ovarian androgenic sensitivity. The factors causing the divergences in response to
androgens are unclear. We postulated that the sensitivities of initial ovaries
are correlated with their gene expression profiles. Before treatment with 17MT,
Japanese eels underwent operation to sample the initial ovarian tissues. The
samples were stored in liquid nitrogen so that the development of ovaries could be followed and traced back to the initial status. Using high-throughput next-generation sequencing
(NGS) technology, ovarian transcriptomic data were mined and analyzed based on
functional gene classification with cutoff-based differentially expressed genes (DEGs). Our results indicated that the gene expression
profiles of initial ovaries correlated with the outcome of 17MT treatment. The ovarian status is embodied to gene expression
profiles globally or is represented by a set of gene list concisely, the initial
ovarian status is a fundament to influence how genotypes translated into
phenotypes by treatments. Our results also implied that neurogenesis or neural activity in the initial ovary might be an
important factor influencing the outcomes of hormonal treatments. In summary, more experiments are required to
support and verify this hypothesis.
Keywords:
Japanese eel, ovarian status, androgens, NGS, gene expression profile
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