一種經濟而快速精準度高的日本鰻 (Anguilla
japonica)
性別判斷方法
黃永森*、羅玟晴、洪銘駿、曾子軒
國立高雄大學 生命科學系
*Email
: yshuang@nuk.edu.tw
摘要:
鰻魚的性別判斷一直是鰻魚人工催熟所遇到的第一個問題，固然學術報告與現場經驗指出，鰻魚的性別可從體型大小、與胸鰭型態判斷，但此原則並不適用於養殖鰻魚。中外學者企圖用分子標記或生理反應的差異來判別公母鰻魚，雖有成果但還有大段的努力空間，最大的缺點是需要時間與實驗室設備。本實驗室曾嘗試用外科手段判別公母，效果良好，但因切口較大而傷口復原需要至少2週以上的時間，期間還可能有感染的問題。今本實驗室利用商用 "採耳棒內視鏡" 配合小切口，直接以目視方式判斷性腺型態以分別公母，效果良好，且價格低廉，因其具防水效能可視為小動物廉價的內視鏡。雖然此方法擁有以上優點且直接快速，但其最大的缺點是，影像清晰度易受液體影響，正進行改良中。
關鍵字 : 鰻魚、人工繁殖、性別分辨、卵巢、內視鏡
An economical, fast and accurate method for sex identification in Japanese
eel (Anguilla japonica)
Yung-Sen Huang*, Wen-Ching Luo, Ming-Chun Hung,
Zih-Syuan Zeng
*email: yshuang@nuk.edu.tw
Abstract
To identify
the sex is the first problem in the artificial maturation of eels. Although
several reports and manipulation experience point out that the sex of eels can
be identified from the body size and the morphology of the pectoral fins, the
criteria do not always apply to aquacultured eels. Scientist have tried to use
molecular biomarkers or differences in physiological responses between two sex
of eels to distinguish sex. Although they have already achieved, there are still
some points to be elucidated, anyways, the major disadvantages are that it
takes time and laboratory equipment is demanded. We have used surgical operation
to identify the sex in eels, the results have been published. However, due to
the large incision, it takes at least 2 weeks to recover, and there may be
infection problems. We employ a commercial "ear picker endoscope"
with a small incision to directly watch the gonads to identify the sex. The
results are impress and the cost is acceptable, it can be regarded as a cheap
endoscope for small animals. Although this method has the advantages, its major
disadvantage of this method is that the image resolution is easily affected by
liquid although its micro camera is waterproof, and it is being improved.
Key word:
Japanese eel, artificial maturation, ovary, sex identification, endoscope
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