Different forms lead to differential results on cellular distributions and functions?

We have clonned two forms of PTEN from Japanese eel, used specific primer, we can tell their different expression levels as well as the different regulatory factors to this difference in vitro. Our results suggest that the change of expression level to the long form may be related to the application of external stimuli, so the expression level of the long one is regulatable, while the expression level is more constant to the short one. we speculate these observations that the long one is pertained to signal responses, and the short one is involved in the integrity of chromosome as some reports indicated (over explaination?). This speculation is reforced by our cell immunofluorescent staining, we used different first serum induced by different recombinant PTEN proteins from rabbit.

so, could you distinguish which picture is stained by the long or the short one ?

A


B