E2 and its metabolite, E1, are rapidly removed from eel muscle tissue reaching background levels within a week after initial treatment with E2. This information could become valuable in the commercial industry for the approval of fish destined to the food market.
25 mg E2/Kg feed. Eels (Anguilla rostrata) of 8 g starting size (9 months post-glass eel) were fed either the control or E2 treated diet at between 3 and 3.5% of body weight per day for 84 days..
https://onlinelibrary.wiley.com/doi/full/10.1002/rcm.7853
為雌化鰻魚，鰻線餵以雌二醇 （Ｅ2，25 mg E2/Kg feed) 達84天。在停餵一週後，鰻魚肌肉組織中的E2和其代謝物 (雌一醇，E1）會快速下降達到背景水平，甚至比野生鰻的還低。 這報告對於食品市場的魚類有參考價值。
Rapid Commun Mass Spectrom. 2017 May 30;31(10):842-850. doi: 10.1002/rcm.7853.
Analysis of 17β-estradiol, estriol and estrone in American eel (Anguilla rostrata) tissue samples using liquid chromatography coupled to electrospray differential ion mobility tandem mass spectrometry.
Abstract
RATIONALE:
17β-Estradiol (E2), estrone (E1) and estriol (E3) are steroid hormones responsible for the regulation of the female reproductive system. Estradiol is planned to be used to feminize eels in aquaculture in order to improve their size and marketability. The residual levels of these hormones in fish tissue must be monitored to meet the requirements of food regulatory agencies. Few studies have studied these hormones in complex biological matrices such as fish tissue.
METHODS:
We developed a method to analyze E1, E2 and E3 in fish tissue using liquid chromatography in combination with differential ion mobility spectrometry (DMS) and tandem mass spectrometry (MS/MS). The mass spectrometer was operated in negative polarity selected reaction monitoring (SRM) mode. To test the performance of this method, residual levels of E1, E2 and E3 were measured in the muscle tissue of juvenile eels subjected to feminization treatment with E2.
RESULTS:
We report that following 17β-estradiol treatment, E2 is rapidly metabolized from the eel tissue, with a 50% depletion rate per day. Five days post-treatment, E2 returned to the level found in non-treated controls, similar to levels found in wild mature female eels.
CONCLUSIONS:
The method presented herein allows the quantitative analysis of E1, E2 and E3 in fish tissue samples. Under the experimental conditions, E2 in fish tissue samples returned to physiological levels post hormonal treatment. Copyright © 2017 John Wiley & Sons, Ltd.
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