2016 Summer Workshop, NTOU (Taiwan) - BOREA (France)
Development, reproduction and evolution in marine organisms
Monday July 25 - Friday July 27, 2016
Effects
of PDE inhibitors on the induced ovarian development in the Japanese eel
1Department of Life
Science, National University of Kaohsiung, Kaohsiung City, Taiwan.2Department
of Aquaculture, National Taiwan Ocean University, Keelung City, Taiwan.
e-mail:
yshuang@.nuk.edu.tw
It is well established
that the reproduction in the Mammal is controlled by the HPG axis, and the
development of gonads is dictated mainly by gonadotropins (GtH, FSH and LH) in
a spatial- and temporal- dependent manner. But, this concept seems
controversial in eels (Anguilla spp.),
since GtH (hCG, PMSG, rFSH, or rLH) alone only stimulates ovarian development
slightly. While the complete ovarian, but not testicular one, development can
be stimulated by pituitaries extracts (PEX) in the eel. Indeed, ovarian FSH- or
LH-receptor has been investigated in the eel during artificial maturation,
these ovarian receptors are present and can be induced by PEX. Both FSH- and
LH-receptor belongs the large family of G protein-coupled receptors (GPCRs), GPCR
binds to and is activated by a specific ligand stimulus (hormones, metabolites
etc.) to produce cAMP by activating adenylyl cyclase (AC), while the cAMP
levels are hydrolyzed into AMP by phosphodiesterase (PDE), both AC and PDE
controls cellular levels of cAMP. So the applications of PDE inhibitors seem to
augment the stimulatory effects of PEX. The aims of our studies are: 1. to investigate
how many different types of PDE in the eel ovary; 2. The applications of
different PDE inhibitors how to effect the stimulatory effects of PEX; 3.the
applications of different PDE inhibitors how to influence the gene expressions
of various ovarian PDE. Our preliminary data indicated that there are at least 5
isotypes of PDE in eel ovary, PDE3 as well as PDE4 effects induced artificial
ovarian development, the PDE on eel ovarian development seems in a spatial-
and temporal- dependent manner.
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