Cloning of Angiopoietin-1

暑假第二篇!
很難達成三篇的目標, 加油
Cloning of Angiopoietin-1(Angpt1), and investigating of expression profiles of VEGF-Flk, and Angpt1 during the development of gas glands in Japanese eel (Anguilla japonica)

Abstract
Angiogenesis is a highly regulated physiological process in animals. Angiopoietin-1 (Angpt1) induces the signaling pathways related to vessel maturation in late phase of angiogenesis, which recruit pericytes supplements to make compact interaction with vessel tubes. There is only few data to show Angpt1 functions in fish. Used degenerate primers, partial sequence (812 bp) of Angpt1 was cloned from Anguilla japonica, deduced amino acids showed 80% similarity with that of zebrafish. Physiological functions of cloned eel Angpt1 were confirmed by in vitro and in vivo manipulations, gas glands (rete mirabile) were the tested target tissue, RT-PCR and immunofluorescent staining techniques were performed, the expression patterns of Angpt1 as well as VEGF-Flk were followed. Data showed that, in vitro, bFGF, PPAR beta agonist, and estradiol obviously effected Angpt1 expression, cobalt ions, a VEGF expression-inducer, did not effect Angpt1 expression. In vivo, expression levels of Angpt1 increase with the body growth. Furthermore, Angpt1 expression was significantly stimulated in the late stage of stimulated gas glands, a succession expression patterns on VEGF-Flk, and Angpt1 on the different development stages of gas glands were observed. Our results suggest that the original function of angiopoietin-1 on the angiogenesis is conserved during the Evolution.


Keywords: angiopoietin-1, angiogenesis, Anguilla, endothelial cells, Flk, gas glands, insulin, rete mirabile, VEGF