PPARs, ACC2, body weight, and lipid content in eels

Association between proliferators activated receptors (PPARs) and acetyl-CoA carboxylase2 (ACC2) expression in muscle tissues or liver in Japanese eel, Anguilla japonica, during growth as well as starvation

Abstract:

        PPARs (peroxisome proliferator-activated receptors) play a key role in lipid metabolism. ACC (acetyl-CoA carboxylase) catalyzes a key intermediates in lipid synthesis. The biosynthesis and catabolism of fatty acid involve equivalent pathways in mammals and in fish, eel (Anguilla spp.), a primitive vertebrate, can store lipids exceed 20% of body weight and swim for a long distance with fasting, it is a good model to study the functions of PPARs and ACC2, these genes are not yet characterized in Japanese eel. So the aims of this report are to investigate different PPARs and ACC2 expressions under different physiological status, and to study the correlation between tissue lipid content and the genes expressions. Our results, evaluated by RT-PCR, qRT-PCR, and tissues lipid content, indicated that: 1). the “saturation” value to lipid content was about 25 %. 2). A lipogenic role of PPAR and lipolysic role of PPAR was showed in eels. 3). PPAR maybe also play permissive roles, but PPAR was believed to have lipolysic functions in the fasted eels, 4). A close association between PPAR and ACC2 expression was found. 5). by ACC2 expression levels, fatty acid synthesis seemed with a constant rate in growing eels, but reduction in fasted eels. All of them support PPARs as well as ACC2 functions on lipid metabolism in eels.

Key words: ACC2, Anguilla japonica, correlation, growth, lipid content, PPARs, starvation