各為鄉親鰻魚除了可蒲燒、清燉外，還可上Cell...
前一篇2009在 Fish Sci., 2013 上 Cell
一閃一閃 亮晶晶 ...(http://pimpeneaucity.blogspot.tw/2009/11/blog-post_17.html)A novel fluorescent protein purified from eel muscle
(by Seiichi Hayashi1, and Yoshifumi Toda, from Fish Sci.,2009(75):1461-1469.)
(by Seiichi Hayashi1, and Yoshifumi Toda, from Fish Sci.,2009(75):1461-1469.)
Cell, 13 June 2013
Copyright © 2013 Elsevier Inc. All rights reserved.
10.1016/j.cell.2013.05.038
A bilirubin-inducible flurorescent protein from eel muscle
Highlights- A novel eel fluorescent protein UnaG belongs to the fatty-acid-binding protein family
- UnaG fluorescence is induced by a noncovalent ligand, bilirubin, a heme metabolite
- The HoloUnaG crystal structure at 1.2 Å revealed a biplanar coordination of bilirubin
- UnaG enabled the establishment of a human bilirubin assay with clinical application
Summary:
fluorescent protein toolbox has revolutionized experimental biology. Despite this advance, no fluorescent proteins have been identified from vertebrates, nor has chromogenic ligand-inducible activation or clinical utility been demonstrated. Here, we report the cloning and characterization of UnaG, a fluorescent protein from Japanese eel. UnaG belongs to the fatty-acid-binding protein (FABP) family, and expression in eel is restricted to small-diameter muscle fibers. On heterologous expression in cell lines or mouse brain, UnaG produces oxygen-independent green fluorescence. Remarkably, UnaG fluorescence is triggered by an endogenous ligand, bilirubin, a membrane-permeable heme metabolite and clinical health biomarker. The holoUnaG structure at 1.2 Å revealed a biplanar coordination of bilirubin by reversible π-conjugation, and we used this high-affinity and high-specificity interaction to establish a fluorescence-based human bilirubin assay with promising clinical utility. UnaG will be the prototype for a versatile class of ligand-activated fluorescent proteins, with applications in research, medicine, and bioengineering.
据日本《产经新闻》6月14日报道，日本理化学研究所研究团队发现，鳗鱼肌肉中的某种蛋白质与血液中的“胆红素”相结合可以发出绿色荧光。该结果已于13日发表在美国科学杂志《CELL》电子版上。
据透露，该项成果很可能会应用于对肝功能以及黄疸的高精度检查中。研究团队把鳗鱼命名为“UnaG”,但在鳗鱼体内如何运行尚未清楚。
研究团队队长宫协敦史表示：“如果可以准确测量胆红素含量，将有助于预防新生儿胆红素脑病。另外，在癌症手术中，也有可能使需切除的癌细胞发光。”
研究团队研究了鳗鱼体内的5条小鱼，发现了形成荧光蛋白质的遗传基因。该蛋白质与血液成分起反应后发出绿色荧光，经检测，起反应的成分为胆红素。
fluorescent protein toolbox has revolutionized experimental biology. Despite this advance, no fluorescent proteins have been identified from vertebrates, nor has chromogenic ligand-inducible activation or clinical utility been demonstrated. Here, we report the cloning and characterization of UnaG, a fluorescent protein from Japanese eel. UnaG belongs to the fatty-acid-binding protein (FABP) family, and expression in eel is restricted to small-diameter muscle fibers. On heterologous expression in cell lines or mouse brain, UnaG produces oxygen-independent green fluorescence. Remarkably, UnaG fluorescence is triggered by an endogenous ligand, bilirubin, a membrane-permeable heme metabolite and clinical health biomarker. The holoUnaG structure at 1.2 Å revealed a biplanar coordination of bilirubin by reversible π-conjugation, and we used this high-affinity and high-specificity interaction to establish a fluorescence-based human bilirubin assay with promising clinical utility. UnaG will be the prototype for a versatile class of ligand-activated fluorescent proteins, with applications in research, medicine, and bioengineering.
据日本《产经新闻》6月14日报道，日本理化学研究所研究团队发现，鳗鱼肌肉中的某种蛋白质与血液中的“胆红素”相结合可以发出绿色荧光。该结果已于13日发表在美国科学杂志《CELL》电子版上。
据透露，该项成果很可能会应用于对肝功能以及黄疸的高精度检查中。研究团队把鳗鱼命名为“UnaG”,但在鳗鱼体内如何运行尚未清楚。
研究团队队长宫协敦史表示：“如果可以准确测量胆红素含量，将有助于预防新生儿胆红素脑病。另外，在癌症手术中，也有可能使需切除的癌细胞发光。”
研究团队研究了鳗鱼体内的5条小鱼，发现了形成荧光蛋白质的遗传基因。该蛋白质与血液成分起反应后发出绿色荧光，经检测，起反应的成分为胆红素。
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